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NEK3 antibody (N-Term)

The Rabbit Polyclonal anti-NEK3 antibody has been validated for WB. It is suitable to detect NEK3 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio).
Catalog No. ABIN2783629

Quick Overview for NEK3 antibody (N-Term) (ABIN2783629)

Target

See all NEK3 Antibodies
NEK3 (NIMA related kinase 3 (NEK3))

Reactivity

Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host

  • 46
  • 3
Rabbit

Clonality

  • 48
  • 1
Polyclonal

Conjugate

  • 18
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NEK3 antibody is un-conjugated

Application

  • 42
  • 19
  • 13
  • 13
  • 10
  • 6
  • 4
  • 3
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 8
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Sequence

    FTQMCLGVNH IHKKRVLHRD IKSKNIFLTQ NGKVKLGDFG SARLLSNPMA

    Predicted Reactivity

    Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Yeast: 79%, Zebrafish: 86%

    Characteristics

    This is a rabbit polyclonal antibody against NEK3. It was validated on Western Blot using a cell lysate as a positive control.

    Purification

    Affinity Purified

    Immunogen

    The immunogen is a synthetic peptide directed towards the N terminal region of human NEK3
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator.

    Comment

    Antigen size: 506 AA

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target

    NEK3 (NIMA related kinase 3 (NEK3))

    Alternative Name

    NEK3

    Background

    NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.
    Alias Symbols: HSPK36, MGC29949
    Protein Interaction Partner: HDAC6, NMNAT1, CDCA8, BPGM, VAV2, PRLR, VAV1, CSN2,
    Protein Size: 506

    Molecular Weight

    58 kDa

    Gene ID

    4752

    NCBI Accession

    NM_002498, NP_002489

    UniProt

    P51956
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