NEK3 antibody

Catalog No. ABIN7229960

The Rabbit Polyclonal anti-NEK3 antibody is suitable to detect NEK3 in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for NEK3 antibody (ABIN7229960)

Target: NEK3 (NIMA related kinase 3 (NEK3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NEK3 Antibody

Purpose: Rabbit Anti-NEK3 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of NEK3 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human NEK3 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEK3

Target: NEK3 (NIMA related kinase 3 (NEK3))

Alternative Name: NEK3

Background: Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3NEK3 encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. NIMA Related Kinase 3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 55kD

Gene ID: 4752

UniProt: P51956