PCDHA12 antibody (N-Term)

Catalog No. ABIN2783721

This Rabbit Polyclonal antibody specifically detects PCDHA12 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for PCDHA12 antibody (N-Term) (ABIN2783721)

Target: PCDHA12 (Protocadherin alpha 12 (PCDHA12))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCDHA12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCDHA12 Antibody

Binding Specificity: N-Term

Purpose: PCDHA12 Antibody - N-terminal region

Sequence: EVIVDRPLQV FHVDVEVKDI NDNPPVFRER EQKVPVSESA PLDSHFPLEG

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 83%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PCDHA12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PCDHA12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP49348-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PCDHA12

Target: PCDHA12 (Protocadherin alpha 12 (PCDHA12))

Alternative Name: PCDHA12

Background:

Background Information: PCDHA12 is a potential calcium-dependent cell-adhesion protein. PCDHA12 may be involved in the establishment and maintenance of specific neuronal connections in the brain.This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Gene Name: Protocadherin alpha 12

Alternative Symbols: PCDH-ALPHA12

Protein Name: Protocadherin alpha-12

Molecular Weight: 99kDa

Gene ID: 56137

NCBI Accession: NP_061726

UniProt: Q9UN75