CYP3A4 antibody (Middle Region)

Catalog No. ABIN2783885

The Rabbit Polyclonal anti-CYP3A4 antibody (ABIN2783885) specifically detects CYP3A4 in WB and IHC. The antibody is reactive with Human, Rat, Cow, Dog, Guinea Pig, Rabbit and Sheep samples.

Quick Overview for CYP3A4 antibody (Middle Region) (ABIN2783885)

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP3A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CYP3A4 Antibody

Binding Specificity: Middle Region

Sequence: ERMKESRLKD KQKHRVDFFQ QMIDSQNSKE TKSHKALSDL ELVAQSIIII

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 79%, Human: 100%, Rabbit: 79%, Rat: 79%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against CYP3A43. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CYP3A43

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 504 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CYP3A4

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Alternative Name: CYP3A43

Background: CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, it is unknown whether the enzyme is also involved in xenobiotic metabolism. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing of this gene results in three transcript variants encoding different isoforms.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, it is unknown whether the enzyme is also involved in xenobiotic metabolism. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing of this gene results in three transcript variants encoding different isoforms.
Alias Symbols: MGC119315, MGC119316
Protein Size: 504

Molecular Weight: 58 kDa

Gene ID: 64816

NCBI Accession: NM_022820, NP_073731

UniProt: Q9HB55

Pathways: Steroid Hormone Biosynthesis