ORAI1 antibody (Middle Region)

Catalog No. ABIN2784058

The Rabbit Polyclonal anti-ORAI1 antibody (ABIN2784058) specifically detects ORAI1 in WB. The antibody is reactive with Human, Rat, Horse, Pig, Cow, Dog, Rabbit and Saccharomyces cerevisiae samples.

Quick Overview for ORAI1 antibody (Middle Region) (ABIN2784058)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human, Rat, Horse, Pig, Cow, Dog, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ORAI1 Antibody

Binding Specificity: Middle Region

Purpose: ORAI1 Antibody - middle region

Sequence: IGTLLFLAEV VLLCWVKFLP LKKQPGQPRP TSKPPASGAA ANVSTSGITP

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 93%, Human: 100%, Pig: 100%, Rabbit: 100%, Rat: 86%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against ORAI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ORAI1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8107373

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1

Background:

Background Information: ORAI1 belongs to the Orai family. ORAI1 (CRACM1) is a plasma membrane protein essential for store-operated calcium entry. Defects in ORAI1 are a cause of severe combined immunodeficiency with CRAC channel dysfunction (CRAC-SCID).CRACM1 is a plasma membrane protein essential for store-operated calcium entry (Vig et al., 2006 [PubMed 16645049]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-331 BC075831.1 1-331 332-844 BC013386.1 220-732 845-990 BC075831.1 845-990 991-1490 BG574128.1 52-551 1491-1496 AK027372.1 1235-1240

Gene Name: ORAI calcium release-activated calcium modulator 1

Alternative Symbols: IMD9, TAM2, ORAT1, CRACM1, TMEM142A

Protein Name: Calcium release-activated calcium channel protein 1

Molecular Weight: 33kDa

Gene ID: 84876

NCBI Accession: NP_116179

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling