Surfeit 4 antibody (N-Term)

Catalog No. ABIN2784078

Cited in 1 publication. The Rabbit Polyclonal anti-Surfeit 4 antibody (ABIN2784078) specifically detects Surfeit 4 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig and Zebrafish (Danio rerio) samples.

Quick Overview for Surfeit 4 antibody (N-Term) (ABIN2784078)

Target: Surfeit 4 (SURF4)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Surfeit 4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Surfeit 4 Antibody

Binding Specificity: N-Term

Purpose: SURF4 Antibody - N-terminal region

Sequence: GQNDLMGTAE DFADQFLRVT KQYLPHVARL CLISTFLEDG IRMWFQWSEQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SURF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SURF4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50161-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Surfeit 4 antibody (ABIN2784078)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for Surfeit 4

Target: Surfeit 4 (SURF4)

Alternative Name: SURF4

Background:

Background Information: SURF4 is a conserved integral membrane protein containing multiple putative transmembrane regions. In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). The specific function of this protein has not been determined but its yeast homolog is directly required for packaging glycosylated pro-alpha-factor into COPII vesicles. This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein containing multiple putative transmembrane regions. In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). The specific function of this protein has not been determined but its yeast homolog is directly required for packaging glycosylated pro-alpha-factor into COPII vesicles. This gene uses multiple polyadenylation sites, resulting in transcript length variation. The existence of alternatively spliced transcript variants has been suggested, but their validity has not been determined.

Gene Name: Surfeit 4

Alternative Symbols: ERV29

Protein Name: Surfeit locus protein 4

Molecular Weight: 30kDa

Gene ID: 6836

NCBI Accession: NP_149351

UniProt: O15260