MED4 antibody (Middle Region)

Catalog No. ABIN2784236

The Rabbit Polyclonal anti-MED4 antibody has been validated for WB and IHC. It is suitable to detect MED4 in samples from Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for MED4 antibody (Middle Region) (ABIN2784236)

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivity: Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MED4 Antibody

Binding Specificity: Middle Region

Sequence: EEIIKYAHRI SASNAVCAPL TWVPGDPRRP YPTDLEMRSG LLGQMNNPST

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MED4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 270 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: MED4

Background: TMED4 contains 1 GOLD domain and belongs to the EMP24/GP25L family. The function remains unknown.
Alias Symbols: DRIP36, FLJ10956, HSPC126, RP11-90M2.2, TRAP36, VDRIP, ARC36
Protein Interaction Partner: LRRFIP1, SMARCE1, SMARCD1, SDCBP, FAM13C, TXLNA, AMOT, C1orf216, MAB21L3, FRMD6, HAUS1, CCDC101, IFT20, USHBP1, CCDC146, CTNNBL1, CCHCR1, TNFAIP8, FTCD, NDC80, SSX3, KIFC3, AES, NDE1, UBC, RPA3, RPA2, RPA1, CDK19, CDK8, MED19, MED26, ESR2, EPAS1, FBXW7, M
Protein Size: 270

Molecular Weight: 30 kDa

Gene ID: 29079

NCBI Accession: NM_014166, NP_054885

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha