YBX1 antibody (C-Term)

Catalog No. ABIN2784387

The Rabbit Polyclonal anti-YBX1 antibody (ABIN2784387) specifically detects YBX1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Sheep, Dog, Horse, Guinea Pig and Pig samples.

Quick Overview for YBX1 antibody (C-Term) (ABIN2784387)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Dog, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-YBX1 Antibody

Binding Specificity: C-Term

Purpose: YBX1 Antibody - C-terminal region

Sequence: PYGRRPQYSN PPVQGEVMEG ADNQGAGEQG RPVRQNMYRG YRPRFRRGPP

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 93%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against YBX1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human YBX1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50946-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: YBX1

Background:

Background Information: YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. YBX1 binds and stabilizes cytoplasmic mRNA. YBX1 contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. YBX1 binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. YBX1 regulates the transcription of numerous genes. YBX1 promotes separation of DNA strands that contain mismatches or are modified by cisplatin. YBX1 has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). YBX1 may play a role in DNA repair. YBX1 is the component of the CRD-mediated complex that promotes MYC mRNA stability.

Alternative Symbols: YB1, BP-8, CSDB, DBPB, YB-1, CBF-A, CSDA2, EFI-A, NSEP1, NSEP-1, MDR-NF1

Molecular Weight: 35kDa

Gene ID: 4904

NCBI Accession: NP_004550

UniProt: P67809

Pathways: Regulation of Muscle Cell Differentiation