BRK1 antibody (Middle Region)

Catalog No. ABIN2784463

The Rabbit Polyclonal anti-BRK1 antibody (ABIN2784463) specifically detects BRK1 in IHC and WB. The antibody is reactive with Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Horse and Pig samples.

Quick Overview for BRK1 antibody (Middle Region) (ABIN2784463)

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-BRK1 Antibody

Binding Specificity: Middle Region

Sequence: YIEIITSSIK KIADFLNSFD MSCRSRLATL NEKLTALERR IEYIEARVTK

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against C3orf10. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human C3orf10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 75 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BRK1

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Alternative Name: C3orf10

Background: C3orf10 is involved in regulation of actin and microtubule organization. It is a part of a WAVE complex that activates the Arp2/3 complex.
Alias Symbols: HSPC300, MDS027, hHBrk1, C3orf10
Protein Interaction Partner: DTNBP1, ACTBL2, NCKAP1, CDKN2A, ACTG1, ACTA1, UBC, PFDN1, CYFIP2, WASF1, NCK1,
Protein Size: 75

Molecular Weight: 9 kDa

Gene ID: 55845

NCBI Accession: NM_018462, NP_060932

UniProt: Q8WUW1

Pathways: RTK Signaling