TAF3 antibody (Middle Region)

Catalog No. ABIN2784481

This Rabbit Polyclonal antibody specifically detects TAF3 in WB. It exhibits reactivity toward Human, Rat, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae.

Quick Overview for TAF3 antibody (Middle Region) (ABIN2784481)

Target: TAF3 (TAF3 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 140kDa (TAF3))

Reactivity: Human, Rat, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TAF3 Antibody

Binding Specificity: Middle Region

Purpose: TAF3 Antibody - middle region

Sequence: RDREREKDKN KDKSKEKDKV KEKEKDKETG RETKYPWKEF LKEEEADPYK

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 92%, Human: 100%, Rabbit: 92%, Rat: 92%, Yeast: 79%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against TAF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TAF3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51249-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TAF3

Target: TAF3 (TAF3 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 140kDa (TAF3))

Alternative Name: TAF3

Background:

Targeted Subunit: 3

Background Information: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 is required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Gene Name: TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140 kDa

Alternative Symbols: TAF140, TAFII140, TAFII-140

Protein Name: Transcription initiation factor TFIID subunit 3

Molecular Weight: 103kDa

Gene ID: 83860

NCBI Accession: NP_114129

UniProt: Q5VWG9

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process, Maintenance of Protein Location