TNNT3 antibody (N-Term)

Catalog No. ABIN2784497

Cited in 2+ publications. The Rabbit Polyclonal anti-TNNT3 antibody (ABIN2784497) specifically detects TNNT3 in WB. The antibody is reactive with Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Horse, Dog, Goat and Zebrafish (Danio rerio) samples.

Quick Overview for TNNT3 antibody (N-Term) (ABIN2784497)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Horse, Dog, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TNNT3 Antibody

Binding Specificity: N-Term

Purpose: TNNT3 Antibody - N-terminal region

Sequence: PRPKLTAPKI PEGEKVDFDD IQKKRQNKDL MELQALIDSH FEARKKEEEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TNNT3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TNNT3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8100501

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-TNNT3 antibody (ABIN2784497)

Zhang, Pereyra, Wang, Birbrair, Reisz, Files, Purcell, Feng, Messi, Feng, Chalovich, Jin, Furdui, Delbono: "Calpain inhibition rescues troponin T3 fragmentation, increases Cav1.1, and enhances skeletal muscle force in aging sedentary mice." in: Aging cell, Vol. 15, Issue 3, pp. 488-98, (2016) (PubMed).

Zhang, Birbrair, Delbono: "Nonmyofilament-associated troponin T3 nuclear and nucleolar localization sequence and leucine zipper domain mediate muscle cell apoptosis." in: Cytoskeleton (Hoboken, N.J.), Vol. 70, Issue 3, pp. 134-47, (2013) (PubMed).

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: TNNT3

Background:

Background Information: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Gene Name: Troponin T type 3 (skeletal, fast)

Alternative Symbols: TNTF, DA2B2, beta-TnTF

Protein Name: Troponin T, fast skeletal muscle

Molecular Weight: 32 kDa

Gene ID: 7140

NCBI Accession: NP_001036245

UniProt: P45378