CDC45 antibody (Cell Division Cycle 45 Homolog (S. Cerevisiae)) (C-Term)

Details for Product anti-CDC45 Antibody No. ABIN2784521
Antigen
  • cdc45l
  • cdc45l2
  • porc-pi-1
  • CDC45L
  • CDC45L2
  • PORC-PI-1
  • Cdc45l
  • cell division cycle 45
  • cell division cycle 45 S homeolog
  • cell division cycle 45 L homeolog
  • cdc45
  • CDC45
  • cdc45.S
  • Cdc45
  • cdc45.L
Epitope
C-Term
10
9
8
7
7
3
3
2
2
2
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
96
49
44
2
1
1
1
1
Host
Rabbit
85
12
Clonality
Polyclonal
Conjugate
This CDC45 antibody is un-conjugated
5
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
Western Blotting (WB)
73
35
34
23
13
10
7
4
3
3
2
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CDC45L
Blocking Peptide Blocking peptide for this product available: ABIN974737
Sequence VTVVGIPPET DSSDRKNFFG RAFEKAAEST SSRMLHNHFD LSVIELKAED
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against CDC45L. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others CDC45 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CDC45L (CDC45 Antibody Abstract)
Background CDC45L was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CDC45, CDC45L2, PORC-PI-1, CDC45L
Protein Interaction Partner: CEP55, UBC, FBXO6, DTD1, TOPBP1, CAMK1G, MCM10, TRAPPC3, ORC3, ORC5, CDKN2A, CDKN1A, ALB, MRE11A, CUL3, NEDD8, CDK2, L3MBTL1, HIST1H3A, CCL2, MCM7, MCM4, MCM3, ORC2, POLA1, CDK4, ORC6, ORC1, MCM6,
Protein Size: 566
Molecular Weight 65 kDa
Gene ID 8318
NCBI Accession NM_003504, NP_003495
UniProt O75419
Pathways Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 566 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (C-Term) antibody (ABIN2784521) WB Suggested Anti-CDC45L Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Pos...
Image no. 2 for anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (C-Term) antibody (ABIN2784521) WB Suggested Anti-CDC45L Antibody Titration: 0.5 ug/mlPositive Control: mouse stem cells
Background publications Hoskins, Marcuello, Altes, Marsh, Maxwell, Van Booven, Paré, Culverhouse, McLeod, Baiget: "Irinotecan pharmacogenetics: influence of pharmacodynamic genes." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 6, pp. 1788-96, 2008 (PubMed).

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