TGIF1 antibody (C-Term)

Catalog No. ABIN2784526

This Rabbit Polyclonal antibody specifically detects TGIF1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Pig and Sheep.

Quick Overview for TGIF1 antibody (C-Term) (ABIN2784526)

Target: TGIF1 (TGFB-Induced Factor Homeobox 1 (TGIF1))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGIF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TGIF1 Antibody

Binding Specificity: C-Term

Sequence: GQNTDIQQIA AKNFTDTSLM YPEDTCKSGP STNTQSGLFN TPPPTPPDLN

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 100%, Rat: 86%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against TGIF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TGIF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 401 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TGIF1

Target: TGIF1 (TGFB-Induced Factor Homeobox 1 (TGIF1))

Alternative Name: TGIF1

Background: TGIF1 is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain.The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.
Alias Symbols: HPE4, MGC39747, MGC5066, TGIF
Protein Interaction Partner: MDFI, CTBP2, CTBP1, APP, PML, ELAVL1, HDAC1, A2M, AR, SIN3A, SMAD3, LRIF1, MED31, MAPK3, SMAD2, MAPK1, JUN, HMGB1, EEF1A1, FOXH1, SMAD1, SDAD1, STK16, MKKS,
Protein Size: 401

Molecular Weight: 43 kDa

Gene ID: 7050

NCBI Accession: NM_170695, NP_733796

UniProt: Q15583

Pathways: Retinoic Acid Receptor Signaling Pathway, Chromatin Binding, Tube Formation