PPIA antibody (Middle Region)

Catalog No. ABIN2784530

The Rabbit Polyclonal anti-PPIA antibody (ABIN2784530) specifically detects PPIA in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit and Sheep samples.

Quick Overview for PPIA antibody (Middle Region) (ABIN2784530)

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivity: Human, Rat, Mouse, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPIA Antibody

Binding Specificity: Middle Region

Purpose: PPIA Antibody - middle region

Sequence: TAKTEWLDGK HVVFGKVKEG MNIVEAMERF GSRNGKTSKK ITIADCGQLE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against PPIA. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPIA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8107811

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: PPIA

Background:

Background Information: PPIA is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. PPIA is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a cyclosporin binding-protein. It may play a role in cyclosporin A-mediated immunosuppression. This protein can interact with several HIV proteins including p55 gag, Vpr, and capsid protein. It has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. Three alternatively spliced transcript variants encoding two distinct isoforms have been observed.

Gene Name: Peptidylprolyl isomerase A (cyclophilin A)

Alternative Symbols: CYPA, CYPH, HEL-S-69p

Protein Name: Peptidyl-prolyl cis-trans isomerase A

Molecular Weight: 18kDa

Gene ID: 5478

NCBI Accession: NP_066953

UniProt: P62937