ABAT antibody (Middle Region)

Catalog No. ABIN2784614

The Rabbit Polyclonal anti-ABAT antibody is suitable to detect ABAT in samples from Human, Mouse, Rat, Dog, Cow, Goat, Guinea Pig, Horse and Rabbit. It has been validated for WB and IHC.

Quick Overview for ABAT antibody (Middle Region) (ABIN2784614)

Target: ABAT (4-Aminobutyrate Aminotransferase (ABAT))

Reactivity: Human, Mouse, Rat, Dog, Cow, Goat, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ABAT Antibody

Binding Specificity: Middle Region

Purpose: ABAT Antibody - middle region

Sequence: YRSKERGQRG FSQEELETCM INQAPGCPDY SILSFMGAFH GRTMGCLATT

Predicted Reactivity: Cow: 93%, Dog: 86%, Goat: 77%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ABAT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ABAT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51765-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ABAT

Target: ABAT (4-Aminobutyrate Aminotransferase (ABAT))

Alternative Name: ABAT

Background:

Background Information: 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95 % similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Gene Name: 4-aminobutyrate aminotransferase

Alternative Symbols: GABAT, NPD009, GABA-AT

Protein Name: 4-aminobutyrate aminotransferase, mitochondrial

Molecular Weight: 56kDa

Gene ID: 18

NCBI Accession: NP_065737

UniProt: P80404

Pathways: Monocarboxylic Acid Catabolic Process