DCK antibody (Middle Region)

Catalog No. ABIN2784622

Cited in 1 publication. The Rabbit Polyclonal anti-DCK antibody (ABIN2784622) specifically detects DCK in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig and Zebrafish (Danio rerio) samples.

Quick Overview for DCK antibody (Middle Region) (ABIN2784622)

Target: DCK (Deoxycytidine Kinase (DCK))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCK Antibody

Binding Specificity: Middle Region

Purpose: DCK Antibody - middle region

Sequence: ATPETCLHRI YLRGRNEEQG IPLEYLEKLH YKHESWLLHR TLKTNFDYLQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against DCK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DCK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51775-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DCK antibody (ABIN2784622)

Bieghs, Caers, De Bruyne, Van Valckenborgh, Higginbotham, Vanderkerken, Menu: "The effects of forodesine in murine and human multiple myeloma cells." in: Advances in hematology, Vol. 2010, pp. 131895, (2010) (PubMed).

Target Details for DCK

Target: DCK (Deoxycytidine Kinase (DCK))

Alternative Name: DCK

Background:

Background Information: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Deoxycytidine kinase

Alternative Symbols: MGC117410, MGC138632

Protein Name: Deoxycytidine kinase

Molecular Weight: 30kDa

Gene ID: 1633

NCBI Accession: NP_000779

UniProt: P27707