Dynamin 1 antibody (Middle Region)

Catalog No. ABIN2784624

The Rabbit Polyclonal anti-Dynamin 1 antibody (ABIN2784624) specifically detects Dynamin 1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig and Horse samples.

Quick Overview for Dynamin 1 antibody (Middle Region) (ABIN2784624)

Target: Dynamin 1 (DNM1)

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Dynamin 1 Antibody

Binding Specificity: Middle Region

Purpose: DNM1 Antibody - middle region

Sequence: PPVDDSWLQV QSVPAGRRSP TSSPTPQRRA PAVPPARPGS RGPAPGPPPA

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against DNM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DNM1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51787-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: DNM1

Background:

Background Information: DNM1 is a member of the dynamin subfamily of GTP-binding proteins. The protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: Dynamin 1

Alternative Symbols: DNM, DEE31, EIEE31

Protein Name: Dynamin-1

Molecular Weight: 95kDa

Gene ID: 1759

NCBI Accession: NP_004399

UniProt: P39053

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling