CCT7 antibody (Middle Region)

Catalog No. ABIN2784636

The Rabbit Polyclonal anti-CCT7 antibody (ABIN2784636) specifically detects CCT7 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse, Dog and Saccharomyces cerevisiae samples.

Quick Overview for CCT7 antibody (Middle Region) (ABIN2784636)

Target: CCT7 (Chaperonin Containing TCP1, Subunit 7 (Eta) (CCT7))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse, Dog, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CCT7 Antibody

Binding Specificity: Middle Region

Purpose: CCT7 Antibody - middle region

Sequence: RAIKNDSVVA GGGAIEMELS KYLRDYSRTI PGKQQLLIGA YAKALEIIPR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CCT7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CCT7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51808-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CCT7

Target: CCT7 (Chaperonin Containing TCP1, Subunit 7 (Eta) (CCT7))

Alternative Name: CCT7

Background:

Targeted Subunit: eta

Background Information: CCT7 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date.

Gene Name: Chaperonin containing TCP1, subunit 7 (eta)

Alternative Symbols: CCTH, CCTETA, NIP7-1, TCP1ETA

Protein Name: T-complex protein 1 subunit eta

Molecular Weight: 60kDa

Gene ID: 10574

NCBI Accession: NP_006420

UniProt: Q99832