Gephyrin antibody (N-Term)

Catalog No. ABIN2784653

This Rabbit Polyclonal antibody specifically detects Gephyrin in WB. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for Gephyrin antibody (N-Term) (ABIN2784653)

Target: Gephyrin (GPHN)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gephyrin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Gephyrin Antibody

Binding Specificity: N-Term

Sequence: HDELEDLPSP PPPLSPPPTT SPHKQTEDKG VQCEEEEEEK KDSGVASTED

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GPHN. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GPHN

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 736 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Gephyrin

Target: Gephyrin (GPHN)

Alternative Name: GPHN

Background: GPHN is a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described, however, the full-length nature of all transcript variants is not currently known. This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described, however, the full-length nature of all transcript variants is not currently known.
Alias Symbols: GEPH, GPH, GPHRYN, KIAA1385
Protein Interaction Partner: UBC, PAAF1, BRCC3, PPP4R1, ROCK2, MTA2, SPTAN1, SPATS1, KHDRBS1, GPHN, ACADVL, PCK1, Dynll1, OTUD4, PRPF4, ENAH, GLRB, DYNLL2, GABARAP, MTOR, PIN1, ARHGEF9, TUBA4A, PFN1,
Protein Size: 736

Molecular Weight: 80 kDa

Gene ID: 10243

NCBI Accession: NM_001024218, NP_001019389

UniProt: Q9NQX3

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development