DPH2 antibody (N-Term)

Catalog No. ABIN2784709

The Rabbit Polyclonal anti-DPH2 antibody is suitable to detect DPH2 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig and Horse. It has been validated for WB.

Quick Overview for DPH2 antibody (N-Term) (ABIN2784709)

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DPH2 Antibody

Binding Specificity: N-Term

Sequence: TTGSKMFILG DTAYGSCCVD VLGAEQAGAQ ALIHFGPACL SPPARPLPVA

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DPH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DPH2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 489 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DPH2

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Alternative Name: DPH2

Background: DPH2 gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: DPH2L2
Protein Interaction Partner: UBC, IL17A, BAG3, MMS19,
Protein Size: 489

Molecular Weight: 52 kDa

Gene ID: 1802

NCBI Accession: NM_001384, NP_001375

UniProt: Q9BQC3