CK1 epsilon antibody (N-Term)

Catalog No. ABIN503293

This Rabbit Polyclonal antibody specifically detects CK1 epsilon in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Sheep, Guinea Pig, Rabbit, Horse, Cow, Saccharomyces cerevisiae and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for CK1 epsilon antibody (N-Term) (ABIN503293)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivity: Human, Mouse, Rat, Dog, Sheep, Guinea Pig, Rabbit, Horse, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CK1 epsilon Antibody

Binding Specificity: N-Term

Purpose: CSNK1E Antibody - N-terminal region

Sequence: MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CSNK1E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CSNK1E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51987-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CK1 epsilon antibody (ABIN503293)

Levran, Londono, OHara, Nielsen, Peles, Rotrosen, Casadonte, Linzy, Randesi, Ott, Adelson, Kreek: "Genetic susceptibility to heroin addiction: a candidate gene association study." in: Genes, brain, and behavior, Vol. 7, Issue 7, pp. 720-9, (2009) (PubMed).

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: CSNK1E

Background:

Background Information: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1E can phosphorylate a large number of proteins. CSNK1E participates in Wnt signaling and is the central component of the circadian clock. It may act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. CSNK1E inhibits cytokine-induced granuloytic differentiation.The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

Gene Name: Casein kinase 1, epsilon

Alternative Symbols: CKIe, HCKIE, CKIepsilon

Protein Name: Casein kinase I isoform epsilon

Molecular Weight: 47kDa

Gene ID: 1454

NCBI Accession: NP_001885

UniProt: P49674

Pathways: Hedgehog Signaling, M Phase