TdT antibody (Middle Region)

Catalog No. ABIN2784741

This Rabbit Polyclonal antibody specifically detects TdT in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig and Rabbit.

Quick Overview for TdT antibody (Middle Region) (ABIN2784741)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TdT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TdT Antibody

Binding Specificity: Middle Region

Sequence: LRRYATHERK MILDNHALYD KTKRIFLKAE SEEEIFAHLG LDYIEPWERN

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 91%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DNTT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DNTT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: DNTT

Background: DNTT is the template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Alias Symbols: TDT
Protein Interaction Partner: REL, APOA1, DNTTIP1, UBC, PRPF19, DNTTIP2, PCNA, XRCC6, ABTB2, CUL3,
Protein Size: 509

Molecular Weight: 58 kDa

Gene ID: 1791

NCBI Accession: NM_004088, NP_004079

UniProt: P04053

Pathways: DNA Damage Repair