SAE1 antibody (N-Term)

Catalog No. ABIN2784763

Product Details anti-SAE1 Antibody

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MVEKEEAGGG ISEEEAAQYD RQIRLWGLEA QKRLRASRVL LVGLKGLGAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%, Yeast: 86%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SAE1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SAE1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 346 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1 (SAE1 Products)

Synonyms: AOS1 antibody, HSPC140 antibody, SUA1 antibody, UBLE1A antibody, 2400010M20Rik antibody, 2610044L12Rik antibody, AL033372 antibody, AW743391 antibody, D7Ertd177e antibody, Sua1 antibody, Uble1a antibody, Aos1p antibody, Sua1p antibody, aos antibody, sae2a antibody, uble1a antibody, wu:fa28b04 antibody, zgc:86633 antibody, SUMO1 activating enzyme subunit 1 antibody, SUMO1 activating enzyme subunit 1 L homeolog antibody, SAE1 antibody, Sae1 antibody, sae1.L antibody, sae1 antibody

Background: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
Alias Symbols: AOS1, FLJ3091, HSPC140, SUA1, UBLE1A
Protein Interaction Partner: FUS, USP39, SUMO2, HSPA2, HSPA1A, UBC, TRMT10A, DPP9, RPRD1B, NPLOC4, OSGEP, PANK4, WDR4, CUL5, TPD52L2, SRP14, RANGAP1, PPP2R5E, MCM3, LPP, DHPS, CALU, ADSL, AARS, PDE12, TP53RK, UBE2I, UBA2, BAG3, PTK2, IL3RA, APP, SUMO1, ETV4, BCL6, UBE2E3, SUMO3, SP10
Protein Size: 346

Molecular Weight: 38 kDa

Gene ID: 10055

NCBI Accession: NM_005500, NP_005491

UniProt: Q9UBE0