NET1 antibody (Middle Region)

Catalog No. ABIN2784785

The Rabbit Polyclonal anti-NET1 antibody is suitable to detect NET1 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for NET1 antibody (Middle Region) (ABIN2784785)

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NET1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NET1 Antibody

Binding Specificity: Middle Region

Purpose: NET1 Antibody - middle region

Sequence: AILIIQGVLS DINLKKGESE CQYYIDKLEY LDEKQRDPRI EASKVLLCHG

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 100%, Yeast: 90%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against NET1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NET1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52123-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Alternative Name: NET1

Background:

Background Information: NET1 acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. It may be involved in activation of the SAPK/JNK pathway.

Gene Name: Neuroepithelial cell transforming 1

Alternative Symbols: NET1A, ARHGEF8

Protein Name: Neuroepithelial cell transforming gene 1 EMBL CAI11031.1

Molecular Weight: 62kDa

Gene ID: 10276

NCBI Accession: NP_005854

UniProt: Q5SQI7

Pathways: Neurotrophin Signaling Pathway