TSEN15 antibody (C-Term)

Catalog No. ABIN2784943

The Rabbit Polyclonal anti-TSEN15 antibody is suitable to detect TSEN15 in samples from Human, Mouse, Cow, Dog, Pig, Rat, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for TSEN15 antibody (C-Term) (ABIN2784943)

Target: TSEN15 (tRNA Splicing Endonuclease 15 Homolog (TSEN15))

Reactivity: Human, Mouse, Cow, Dog, Pig, Rat, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSEN15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TSEN15 Antibody

Binding Specificity: C-Term

Purpose: Tsen15 Antibody - C-terminal region

Sequence: ASLSHNRIRE ILKASRKLQG DPELPMSFTL AIVESDSTIV YYKLTDGFML

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Tsen15. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52469-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TSEN15

Target: TSEN15 (tRNA Splicing Endonuclease 15 Homolog (TSEN15))

Alternative Name: Tsen15

Background:

Targeted Subunit: Sen15

Background Information: Tsen15 is a non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

Gene Name: TRNA splicing endonuclease 15 homolog (S. cerevisiae)

Alternative Symbols: Sen15, AL023077, 5730449L18Rik

Protein Name: tRNA-splicing endonuclease subunit Sen15

Molecular Weight: 18kDa

Gene ID: 66637

NCBI Accession: NP_079953

UniProt: Q8R3W5