ADC antibody (C-Term)

Catalog No. ABIN2784945

The Rabbit Polyclonal anti-ADC antibody is suitable to detect ADC in samples from Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Sheep and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for ADC antibody (C-Term) (ABIN2784945)

Target: ADC (Arginine Decarboxylase (ADC))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Sheep, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADC Antibody

Binding Specificity: C-Term

Purpose: AZIN2 Antibody - C-terminal region

Sequence: AELGRYYVTS AFTVAVSIIA KKEVLLDQPG REEENGSTSK TIVYHLDEGV

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 85%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against ADC. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human ADC

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52477-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ADC

Target: ADC (Arginine Decarboxylase (ADC))

Alternative Name: AZIN2

Background:

Background Information: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene.

Gene Name: antizyme inhibitor 2

Alternative Symbols: ADC, AZI2, ODCp, AZIB1, ODC-p, ODC1L

Protein Name: antizyme inhibitor 2

Molecular Weight: 41kDa

Gene ID: 113451

NCBI Accession: NP_443724

UniProt: Q96A70