RDH12 antibody (Middle Region)

Catalog No. ABIN2785175

There is 1 publication for this product available. The Rabbit Polyclonal anti-RDH12 antibody is suitable to detect RDH12 in samples from Human, Pig, Rat and Dog. It has been validated for WB.

Quick Overview for RDH12 antibody (Middle Region) (ABIN2785175)

Target: RDH12 (Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12))

Reactivity: Human, Pig, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RDH12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RDH12 Antibody

Binding Specificity: Middle Region

Purpose: RDH12 Antibody - middle region

Sequence: HIGKIPFHDL QSEKRYSRGF AYCHSKLANV LFTRELAKRL QGTGVTTYAV

Predicted Reactivity: Dog: 93%, Human: 100%, Pig: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against RDH12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RDH12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52972-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RDH12 antibody (ABIN2785175)

Sun, Gerth, Maeda, Lodowski, Van Der Kraak, Saperstein, Héon, Palczewski: "Novel RDH12 mutations associated with Leber congenital amaurosis and cone-rod dystrophy: biochemical and clinical evaluations." in: Vision research, Vol. 47, Issue 15, pp. 2055-66, (2007) (PubMed).

Target Details for RDH12

Target: RDH12 (Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12))

Alternative Name: RDH12

Background:

Background Information: RDH12 is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 3 (LCA3).The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 3 (LCA3).

Gene Name: Retinol dehydrogenase 12 (all-trans/9-cis/11-cis)

Alternative Symbols: RP53, LCA13, SDR7C2

Protein Name: Retinol dehydrogenase 12

Molecular Weight: 35kDa

Gene ID: 145226

NCBI Accession: NP_689656

UniProt: Q96NR8