RDH12 antibody (Middle Region)

Catalog No. ABIN2785176

The Rabbit Polyclonal anti-RDH12 antibody is suitable to detect RDH12 in samples from Human, Pig, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for RDH12 antibody (Middle Region) (ABIN2785176)

Target: RDH12 (Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12))

Reactivity: Human, Pig, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RDH12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RDH12 Antibody

Binding Specificity: Middle Region

Purpose: RDH12 Antibody - middle region

Sequence: AKRLQGTGVT TYAVHPGVVR SELVRHSSLL CLLWRLFSPF VKTAREGAQT

Predicted Reactivity: Cow: 85%, Dog: 92%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Pig: 86%, Rabbit: 85%, Rat: 79%, Yeast: 79%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RDH12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RDH12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52973-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RDH12

Target: RDH12 (Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12))

Alternative Name: RDH12

Background:

Background Information: RDH12 is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 3 (LCA3). The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 3 (LCA3).

Gene Name: Retinol dehydrogenase 12 (all-trans/9-cis/11-cis)

Alternative Symbols: RP53, LCA13, SDR7C2

Protein Name: Retinol dehydrogenase 12

Molecular Weight: 35kDa

Gene ID: 145226

NCBI Accession: NP_689656

UniProt: Q96NR8