ENPP6 antibody (Middle Region)

Catalog No. ABIN2785260

The Rabbit Polyclonal anti-ENPP6 antibody is suitable to detect ENPP6 in samples from Human, Mouse, Rat, Cow, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Dog and Guinea Pig. It has been validated for WB.

Quick Overview for ENPP6 antibody (Middle Region) (ABIN2785260)

Target: ENPP6 (Ectonucleotide pyrophosphatase/phosphodiesterase 6 (ENPP6))

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENPP6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ENPP6 Antibody

Binding Specificity: Middle Region

Purpose: ENPP6 Antibody - middle region

Sequence: ELMDMRGIFL AFGPDFKSNF RAAPIRSVDV YNVMCNVVGI TPLPNNGSWS

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ENPP6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ENPP6

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53158-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ENPP6

Target: ENPP6 (Ectonucleotide pyrophosphatase/phosphodiesterase 6 (ENPP6))

Alternative Name: ENPP6

Background:

Background Information: ENPP6 is a choline-specific glycerophosphodiester phosphodiesterase. ENPP6 hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. ENPP6 hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. ENPP6 has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently.

Gene Name: Ectonucleotide pyrophosphatase/phosphodiesterase 6

Alternative Symbols: NPP6

Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 6

Molecular Weight: 50kDa

Gene ID: 133121

NCBI Accession: NP_699174

UniProt: Q6UWR7