EIF5 antibody (N-Term)

Catalog No. ABIN2785396

This Rabbit Polyclonal antibody specifically detects EIF5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Guinea Pig, Dog, Rabbit and Zebrafish (Danio rerio).

Quick Overview for EIF5 antibody (N-Term) (ABIN2785396)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reactivity: Human, Mouse, Rat, Cow, Horse, Guinea Pig, Dog, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF5 Antibody

Binding Specificity: N-Term

Purpose: EIF5 Antibody - N-terminal region

Sequence: SVNVNRSVSD QFYRYKMPRL IAKVEGKGNG IKTVIVNMVD VAKALNRPPT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53443-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: EIF5

Background:

Background Information: EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Gene Name: Eukaryotic translation initiation factor 5

Alternative Symbols: EIF-5, EIF-5A

Protein Name: Eukaryotic translation initiation factor 5

Molecular Weight: 49kDa

Gene ID: 1983

NCBI Accession: NP_892116

UniProt: P55010