CRISP1 antibody (N-Term)

Catalog No. ABIN2785458

The Rabbit Polyclonal anti-CRISP1 antibody (ABIN2785458) specifically detects CRISP1 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CRISP1 antibody (N-Term) (ABIN2785458)

Target: CRISP1 (Cysteine-Rich Secretory Protein 1 (CRISP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRISP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRISP1 Antibody

Binding Specificity: N-Term

Purpose: CRISP1 Antibody - N-terminal region

Sequence: ARIFSKYCDM TESNPLERRL PNTFCGENMH MTSYPVSWSS VIGVWYSEST

Predicted Reactivity: Human: 100%, Mouse: 77%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against CRISP1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP53581-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CRISP1

Target: CRISP1 (Cysteine-Rich Secretory Protein 1 (CRISP1))

Alternative Name: CRISP1

Background:

Background Information: Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. CRISP1 is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface.

Gene Name: Cysteine-rich secretory protein 1

Alternative Symbols: ARP, AEGL1, HUMARP, CRISP-1, HEL-S-57, HSCRISP1D, HSCRISP1G

Protein Name: Cysteine-rich secretory protein 1

Molecular Weight: 27kDa

Gene ID: 167

NCBI Accession: NP_001122

UniProt: P54107