PRKAA1 antibody (Middle Region)

Catalog No. ABIN2785500

The Rabbit Polyclonal anti-PRKAA1 antibody (ABIN2785500) specifically detects PRKAA1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio) and Sheep samples.

Quick Overview for PRKAA1 antibody (Middle Region) (ABIN2785500)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKAA1 Antibody

Binding Specificity: Middle Region

Sequence: SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PRKAA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRKAA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 559 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1

Background: PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: AMPK, AMPKa1, MGC33776, MGC57364
Protein Interaction Partner: PRKAG1, PRKAB2, PRKAB1, GOLGA2, PHC2, CTBP1, CDX4, AES, Trp53, INO80E, RFX6, TRIM27, KRT40, SSX2IP, PNMA5, UBXN11, RIMBP3, L3MBTL3, TSC22D4, VPS37B, HMBOX1, HOMEZ, THAP1, ROPN1, RAD54B, MTUS2, IKZF3, RBPMS, ABI2, ZBED1, BHLHE40, TRIP6, STK11, VPS52, PPARG
Protein Size: 559

Molecular Weight: 63 kDa

Gene ID: 5562

NCBI Accession: NM_006251, NP_006242

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect