PRKAA1 antibody (N-Term)

Catalog No. ABIN2785614

The Rabbit Polyclonal anti-PRKAA1 antibody has been validated for WB and IHC. It is suitable to detect PRKAA1 in samples from Human, Rat and Rabbit.

Quick Overview for PRKAA1 antibody (N-Term) (ABIN2785614)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRKAA1 Antibody

Binding Specificity: N-Term

Sequence: GELFDYICKN GRKSDVPGVV KTGSTKELDE KESRRLFQQI LSGVDYCHRH

Predicted Reactivity: Human: 100%, Rabbit: 85%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against PRKAA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRKAA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 565 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1

Background: PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: AMPK, AMPKa1, MGC33776, MGC57364
Protein Interaction Partner: PRKAG1, PRKAB2, PRKAB1, GOLGA2, PHC2, CTBP1, CDX4, AES, Trp53, INO80E, RFX6, TRIM27, KRT40, SSX2IP, PNMA5, UBXN11, RIMBP3, L3MBTL3, TSC22D4, VPS37B, HMBOX1, HOMEZ, THAP1, ROPN1, RAD54B, MTUS2, IKZF3, RBPMS, ABI2, ZBED1, BHLHE40, TRIP6, STK11, VPS52, PPARG
Protein Size: 565

Molecular Weight: 62 kDa

Gene ID: 5562

NCBI Accession: NM_206907, NP_996790

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect