ATG3 antibody (Middle Region)

Catalog No. ABIN2785623

Product Details anti-ATG3 Antibody

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LWLFGYDEQR QPLTVEHMYE DISQDHVKKT VTIENHPHLP PPPMCSVHPC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ATG3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATG3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 314 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3 (ATG3 Products)

Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).
Alias Symbols: APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Protein Interaction Partner: HUWE1, MAP1LC3B, UBC, SUMO1, NEDD8, ATG7, ATG5, ATG12, BECN1, HSPA9, DFFA, ASS1, NHLRC2, NANS, TNFAIP8, RPL23, BAG3, FNBP1L, GABARAPL1, MAP1LC3A, CFLAR, CREBBP, MAP1LC3C, ATG10, AMBRA1, ATG16L1, TECPR1, OBSL1, ATG4B, GABARAPL2, GABARAP, MAP1B, KRT85,
Protein Size: 314

Molecular Weight: 36 kDa

Gene ID: 64422

NCBI Accession: NM_022488, NP_071933

UniProt: Q9NT62

Pathways: Autophagy