ATG3 antibody (AA 1-314)

Catalog No. ABIN7174567

Product Details anti-ATG3 Antibody

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Binding Specificity: AA 1-314

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Ubiquitin-like-conjugating enzyme ATG3 protein (1-314AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3 (ATG3 Products)

Synonyms: atg3 antibody, im:6910145 antibody, wu:fb15g12 antibody, zgc:64156 antibody, APG3 antibody, APG3-LIKE antibody, APG3L antibody, PC3-96 antibody, 2610016C12Rik antibody, Apg3l antibody, Atg3l antibody, PIG-1 antibody, Pig1 antibody, ATATG3 antibody, K11J9.1 antibody, K11J9_1 antibody, autophagy related 3 antibody, autophagy 3 (APG3) antibody, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy antibody, atg3 antibody, ATG3 antibody, Atg3 antibody

Background:

Background: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Aliases: 2610016C12Rik antibody, Apg 3 antibody, APG3 antibody, APG3 autophagy 3 like antibody, APG3 like antibody, APG3, S. cerevisiae, homolog of antibody, APG3-like antibody, APG3L antibody, Apg3p antibody, ATG 3 antibody, ATG3 antibody, ATG3 autophagy related 3 homolog antibody, ATG3 autophagy related 3 homolog (S. cerevisiae) antibody, ATG3_HUMAN antibody, Autophagy 3, S. cerevisiae, homolog of antibody, Autophagy Apg3p/Aut1p like antibody, autophagy related 3 antibody, Autophagy related protein 3 antibody, Autophagy-related protein 3 antibody, DKFZp564M1178 antibody, FLJ22125 antibody, hApg3 antibody, MGC15201 antibody, OTTHUMP00000214547 antibody, OTTHUMP00000214548 antibody, PC3 96 antibody, Protein PC3-96 antibody, Ubiquitin-like-conjugating enzyme ATG3 antibody

UniProt: Q9NT62

Pathways: Autophagy