ATG3 antibody (Middle Region)

Catalog No. ABIN2774245

This Rabbit Polyclonal antibody specifically detects ATG3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit.

Quick Overview for ATG3 antibody (Middle Region) (ABIN2774245)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG3 Antibody

Binding Specificity: Middle Region

Purpose: ATG3 Antibody - middle region

Sequence: SDELEAIIEE DDGDGGWVDT YHNTGITGIT EAVKEITLEN KDNIRLQDCS

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ATG3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATG3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59069-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3

Background:

Background Information: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).

Gene Name: ATG3 autophagy related 3 homolog (S. cerevisiae)

Alternative Symbols: APG3, APG3L, PC3-96, APG3-LIKE

Protein Name: Ubiquitin-like-conjugating enzyme ATG3

Molecular Weight: 36kDa

Gene ID: 64422

NCBI Accession: NP_071933

UniProt: Q9NT62

Pathways: Autophagy