ACAT1 antibody (Middle Region)

Catalog No. ABIN2785628

The Rabbit Polyclonal anti-ACAT1 antibody (ABIN2785628) specifically detects ACAT1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Saccharomyces cerevisiae samples.

Quick Overview for ACAT1 antibody (Middle Region) (ABIN2785628)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ACAT1 Antibody

Binding Specificity: Middle Region

Purpose: ACAT1 Antibody - middle region

Sequence: GHQDVMVAGG MESMSNVPYV MNRGSTPYGG VKLEDLIVKD GLTDVYNKIH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ACAT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ACAT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54277-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1

Background:

Background Information: ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The gene encoding ACAT1 spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Gene Name: Acetyl-CoA acetyltransferase 1

Alternative Symbols: T2, MAT, ACAT, THIL

Protein Name: Acetyl-CoA acetyltransferase, mitochondrial

Molecular Weight: 41

Gene ID: 38

NCBI Accession: NP_000010

UniProt: P24752