GCAP1 antibody (N-Term)

Catalog No. ABIN2785648

The Rabbit Polyclonal anti-GCAP1 antibody is suitable to detect GCAP1 in samples from Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Pig and Rabbit. It has been validated for WB.

Quick Overview for GCAP1 antibody (N-Term) (ABIN2785648)

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCAP1 Antibody

Binding Specificity: N-Term

Purpose: GUCA1A Antibody - N-terminal region

Sequence: LYEFRQFFGL KNLSPSASQY VEQMFETFDF NKDGYIDFME YVAALSLVLK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GUCA1A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GUCA1A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54309-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Alternative Name: GUCA1A

Background:

Background Information: GUCA1A(GCAP1) plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3), a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations, causing the constitutive activation of RetGC1 and, presumably, increased cell death. This gene is expressed in retina and spermatagonia.

Gene Name: Guanylate cyclase activator 1A (retina)

Alternative Symbols: COD3, GCAP, GUCA, GCAP1, GUCA1, CORD14, C6orf131

Protein Name: Guanylyl cyclase-activating protein 1

Molecular Weight: 23kDa

Gene ID: 2978

NCBI Accession: NP_000400

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction