Glutathione Reductase antibody (N-Term)

Catalog No. ABIN2785662

The Rabbit Polyclonal anti-Glutathione Reductase antibody has been validated for WB and IHC. It is suitable to detect Glutathione Reductase in samples from Human, Rat, Mouse, Rabbit, Guinea Pig, Cow, Dog and Horse. There is 1 publication available.

Quick Overview for Glutathione Reductase antibody (N-Term) (ABIN2785662)

Target: Glutathione Reductase (GSR)

Reactivity: Human, Rat, Mouse, Rabbit, Guinea Pig, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutathione Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Glutathione Reductase Antibody

Binding Specificity: N-Term

Sequence: PTIEVSGKKY TAPHILIATG GMPSTPHESQ IPGASLGITS DGFFQLEELP

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GSR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GSR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 522 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Glutathione Reductase antibody (ABIN2785662)

Hoffmann, Dietrich, Herrmann, Schacht, Albrecht, Methner: "Dimethyl Fumarate Induces Glutathione Recycling by Upregulation of Glutathione Reductase." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6093903, (2017) (PubMed).

Target Details for Glutathione Reductase

Target: Glutathione Reductase (GSR)

Alternative Name: GSR

Background: GSR belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It maintains high levels of reduced glutathione in the cytosol. Both glutathione and glutathione reductase are inducible by D3T, and that upregulation of GSH biosynthesis underlies D3T-mediated cytoprotection against ROS/RNS-elicited injury to human vascular smooth muscle cells.
Alias Symbols: MGC78522
Protein Interaction Partner: SUMO1, CNDP2, GINS2, PROSC, AHSA1, NDRG1, DDX39A, NAMPT, KYNU, DDX39B, RPS6KA1, PEPD, MVD, LDHA, HSPD1, GBP2, EEF2, ADSS, C11orf49, UBC, FOLR1, ISG15, ELAVL1, SUMO4, PJA1, OCM, GSR,
Protein Size: 522

Molecular Weight: 56 kDa

Gene ID: 2936

NCBI Accession: NM_000637, NP_000628

UniProt: P00390

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis