UBE4B antibody (N-Term)

Catalog No. ABIN2785770

The Rabbit Polyclonal anti-UBE4B antibody (ABIN2785770) specifically detects UBE4B in WB. The antibody is reactive with Human, Dog and Pig samples.

Quick Overview for UBE4B antibody (N-Term) (ABIN2785770)

Target: UBE4B (Ubiquitin Conjugation Factor E4 B (UBE4B))

Reactivity: Human, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE4B Antibody

Binding Specificity: N-Term

Purpose: UBE4B Antibody - N-terminal region

Sequence: SPMFCSVASF GASSLSSLYE SSPAPTPSFW SSVPVMGPSL ASPSRAASQL

Predicted Reactivity: Dog: 79%, Human: 100%, Pig: 79%

Characteristics: This is a rabbit polyclonal antibody against UBE4B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE4B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54552-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE4B

Target: UBE4B (Ubiquitin Conjugation Factor E4 B (UBE4B))

Alternative Name: UBE4B

Background:

Background Information: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B is an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. The gene that encodes the protein is also the strongest candidate in the neuroblastoma tumor suppressor genes. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: Ubiquitination factor E4B

Alternative Symbols: E4, UFD2, HDNB1, UBOX3, UFD2A

Protein Name: Ubiquitin conjugation factor E4 B

Molecular Weight: 146kDa

Gene ID: 10277

NCBI Accession: NP_001099032

UniProt: O95155