GLE1 antibody (N-Term)

Catalog No. ABIN2785781

The Rabbit Polyclonal anti-GLE1 antibody has been validated for WB. It is suitable to detect GLE1 in samples from Human, Rat, Mouse, Horse, Cow, Dog, Pig, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for GLE1 antibody (N-Term) (ABIN2785781)

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Reactivity: Human, Rat, Mouse, Horse, Cow, Dog, Pig, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLE1 Antibody

Binding Specificity: N-Term

Sequence: LKLREAEQQR VKQAEQERLR KEEGQIRLRA LYALQEEMLQ LSQQLDASEQ

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 86%, Rabbit: 77%, Rat: 100%, Yeast: 90%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against GLE1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLE1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 659 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GLE1

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Alternative Name: GLE1

Background: GLE1 is a predicted 75- kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm.This gene encodes a predicted 75- kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: GLE1L, hGLE1, LCCS, LCCS1
Protein Interaction Partner: RNF2, UBC, ELAVL1, NUPL2, NUP155, EIF3F, UXT, KRT10,
Protein Size: 659

Molecular Weight: 75 kDa

Gene ID: 2733

NCBI Accession: NM_001499, NP_001490

UniProt: Q53GS7