GLE1 antibody (N-Term)

Catalog No. ABIN2785781

Product Details anti-GLE1 Antibody

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Horse, Cow, Dog, Pig, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLE1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LKLREAEQQR VKQAEQERLR KEEGQIRLRA LYALQEEMLQ LSQQLDASEQ

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 86%, Rabbit: 77%, Rat: 100%, Yeast: 90%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against GLE1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLE1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 659 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GLE1

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Alternative Name: GLE1 (GLE1 Products)

Background: GLE1 is a predicted 75- kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm.This gene encodes a predicted 75- kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: GLE1L, hGLE1, LCCS, LCCS1
Protein Interaction Partner: RNF2, UBC, ELAVL1, NUPL2, NUP155, EIF3F, UXT, KRT10,
Protein Size: 659

Molecular Weight: 75 kDa

Gene ID: 2733

NCBI Accession: NM_001499, NP_001490

UniProt: Q53GS7