ARF6 antibody (Middle Region)

Catalog No. ABIN2785790

The Rabbit Polyclonal anti-ARF6 antibody has been validated for WB and IHC. It is suitable to detect ARF6 in samples from Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Sheep and Dog. There is 1 publication available.

Quick Overview for ARF6 antibody (Middle Region) (ABIN2785790)

Target: ARF6 (ADP-Ribosylation Factor 6 (ARF6))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Sheep, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ARF6 Antibody

Binding Specificity: Middle Region

Sequence: REMRDAIILI FANKQDLPDA MKPHEIQEKL GLTRIRDRNW YVQPSCATSG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ARF6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARF6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 175 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ARF6 antibody (ABIN2785790)

Tagliatti, Fadda, Falace, Benfenati, Fassio: "Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse." in: eLife, Vol. 5, (2016) (PubMed).

Target Details for ARF6

Target: ARF6 (ADP-Ribosylation Factor 6 (ARF6))

Alternative Name: ARF6

Background: ARF6 is a member of the human ARF family, which is part of the RAS superfamily. They are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF6 is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp564M0264
Protein Interaction Partner: UBC, LSB5, GGA1, STAU1, EGFR, CYTH1, CYTH2, GNAQ, FN1, VCP, APP, RAB11FIP3, RAB11FIP4, RAB11FIP5, USP6, IKBKG, GGA3, ITSN1, ASAP1, FBXO8, MEPCE, RUVBL2, ASAP3, MPP5, ZNF709, EXOC5, ARFIP2, AP3D1, PIP5K1C, ASAP2, AP3B1, PIP5K1A, PLD1, AP1B1, EZR, CHRM3, CA
Protein Size: 175

Molecular Weight: 20 kDa

Gene ID: 382

NCBI Accession: NM_001663, NP_001654

UniProt: P62330

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Actin Filament Polymerization, SARS-CoV-2 Protein Interactome