KPNA3 antibody (N-Term)

Catalog No. ABIN2785825

The Rabbit Polyclonal anti-KPNA3 antibody is suitable to detect KPNA3 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for KPNA3 antibody (N-Term) (ABIN2785825)

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KPNA3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KPNA3 Antibody

Binding Specificity: N-Term

Purpose: KPNA3 Antibody - N-terminal region

Sequence: AENPSLENHR IKSFKNKGRD VETMRRHRNE VTVELRKNKR DEHLLKKRNV

Predicted Reactivity: Cow: 77%, Dog: 77%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against KPNA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KPNA3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54674-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KPNA3

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Alternative Name: KPNA3

Background:

Targeted Subunit: alpha-3

Background Information: The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3 is a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggest that karyopherin alpha-3 may be involved in the nuclear transport system.The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Karyopherin alpha 3 (importin alpha 4)

Alternative Symbols: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma

Protein Name: Importin subunit alpha-3

Molecular Weight: 58kDa

Gene ID: 3839

NCBI Accession: NP_002258

UniProt: O00505

Pathways: Protein targeting to Nucleus