ICA1 antibody (C-Term)

Catalog No. ABIN2785862

This anti-ICA1 antibody is a Rabbit Polyclonal antibody detecting ICA1 in WB. Suitable for Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit. This Primary Antibody has been cited in 1 publication.

Quick Overview for ICA1 antibody (C-Term) (ABIN2785862)

Target: ICA1 (Islet Cell Autoantigen 1, 69kDa (ICA1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ICA1 Antibody

Binding Specificity: C-Term

Sequence: NMKDLQASLQ EPAKAASDLT AWFSLFADLD PLSNPDAVGK TDKEHELLNA

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ICA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ICA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 483 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ICA1 antibody (ABIN2785862)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for ICA1

Target: ICA1 (Islet Cell Autoantigen 1, 69kDa (ICA1))

Alternative Name: ICA1

Background: ICA1 is a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Alternatively spliced variants which encode different protein isoforms have been described, however, not all variants have been fully characterized.
Alias Symbols: ICA69, ICAp69
Protein Interaction Partner: RAB2B, ING5, MBD3, CCDC28A, RAB2A, tbc-8, EXOC5, STK16, MKKS, KRT33B, CNTF,
Protein Size: 483

Molecular Weight: 53 kDa

Gene ID: 3382

NCBI Accession: NM_022307, NP_071682

UniProt: Q05084