14-3-3 sigma/SFN antibody (N-Term)

Catalog No. ABIN2785885

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody has been validated for WB. It is suitable to detect 14-3-3 sigma/SFN in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep and Guinea Pig.

Quick Overview for 14-3-3 sigma/SFN antibody (N-Term) (ABIN2785885)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: N-Term

Sequence: VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against SFN. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SFN

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 248 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN

Background: SFN is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. SFN binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, SFN regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.
Alias Symbols: YWHAS
Protein Interaction Partner: FAM9B, RAB3IP, LONRF1, CCDC102B, ZC2HC1C, DTL, FAM53C, MKRN3, FOXO4, MAGEA1, CCNH, SIK3, ZFYVE19, YWHAZ, VCL, UBE2V1, SNRPF, UBE2R2, PAK2, G6PD, TARDBP, EGFR, BAD, MARK3, HDAC7, HDAC5, HDAC4, RAF1, CDC25C, MST1R, ABL1, HNRNPD, ATF2, TRIM32, COPS6, UBC, TP
Protein Size: 248

Molecular Weight: 28 kDa

Gene ID: 2810

NCBI Accession: NM_006142, NP_006133

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction