GLO1 antibody (N-Term)

Catalog No. ABIN2785894

The Rabbit Polyclonal anti-GLO1 antibody (ABIN2785894) specifically detects GLO1 in WB. The antibody is reactive with Human, Mouse, Rat, Saccharomyces cerevisiae, Guinea Pig, Zebrafish (Danio rerio), Cow, Dog, Horse and Rabbit samples.

Quick Overview for GLO1 antibody (N-Term) (ABIN2785894)

Target: GLO1 (Glyoxalase I (GLO1))

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Guinea Pig, Zebrafish (Danio rerio), Cow, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLO1 Antibody

Binding Specificity: N-Term

Purpose: GLO1 Antibody - N-terminal region

Sequence: TMLRVKDPKK SLDFYTRVLG MTLIQKCDFP IMKFSLYFLA YEDKNDIPKE

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 86%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against GLO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLO1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54803-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: GLO1

Background:

Background Information: The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Gene Name: Glyoxalase I

Alternative Symbols: GLYI, GLOD1, HEL-S-74

Protein Name: Lactoylglutathione lyase

Molecular Weight: 21kDa

Gene ID: 2739

NCBI Accession: NP_006699

UniProt: Q04760