APPL1 antibody (Middle Region)

Catalog No. ABIN2785912

The Rabbit Polyclonal anti-APPL1 antibody is suitable to detect APPL1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for APPL1 antibody (Middle Region) (ABIN2785912)

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APPL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APPL1 Antibody

Binding Specificity: Middle Region

Sequence: GQAKAFGQGG RRTNPFGESG GSTKSETEDS ILHQLFIVRF LGSMEVKSDD

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 75%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against APPL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human APPL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 709 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for APPL1

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Alternative Name: APPL1

Background: The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A
Alias Symbols: APPL, DIP13alpha
Protein Interaction Partner: RHEBL1, UBE2O, APPL2, APPL1, FARS2, MEOX1, MAGEA9, UBC, KIAA1468, CLUH, TTC4, TRAF6, ADIPOR1, HDAC5, HDAC3, HDAC2, PIK3R1, AR, AKT1, CMTM4, PLEKHF2, KXD1, DPYSL5, EGFR, RAB21, VCP, RAB5A, AKT2, ADIPOR2, FSHR, ELAVL1, MTA2, RBBP7, RUVBL2, RBBP4, HDAC1, TRA
Protein Size: 709

Molecular Weight: 80 kDa

Gene ID: 26060

NCBI Accession: NM_012096, NP_036228

UniProt: Q9UKG1