MRPL13 antibody (Middle Region)

Catalog No. ABIN2785985

The Rabbit Polyclonal anti-MRPL13 antibody has been validated for WB. It is suitable to detect MRPL13 in samples from Human, Mouse, Rat, Guinea Pig, Horse, Pig, Cow, Dog, Rabbit, Zebrafish (Danio rerio).

Quick Overview for MRPL13 antibody (Middle Region) (ABIN2785985)

Target: MRPL13 (Mitochondrial Ribosomal Protein L13 (MRPL13))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Pig, Cow, Dog, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL13 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL13 Antibody

Binding Specificity: Middle Region

Sequence: AIYGMLPKNL HRRTMMERLH LFPDEYIPED ILKNLVEELP QPRKIPKRLD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against MRPL13. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MRPL13

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 178 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL13

Target: MRPL13 (Mitochondrial Ribosomal Protein L13 (MRPL13))

Alternative Name: MRPL13

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
Alias Symbols: L13, L13mt, RPL13, RPML13, L13A
Protein Interaction Partner: FN1, MRPL55, MRPL10, MRPL52, MRPL45, MRPL24, MRPL44, MRPL9, MRPL11, MRPL38, MRPL41, MRPS9, MRPL14, MRPL17, MRPL16, MRPL39, MRPL2, MRPL15, MRPL42, MRPS28, SCFD1, MRPL3, CUL7, MRPL19, MRPL23, SLC25A3, OGDH, NDUFS1, NDUFB10, MPV17, ICT1, HNRNPU, DLD, UBC, OX
Protein Size: 178

Molecular Weight: 21 kDa

Gene ID: 28998

NCBI Accession: NM_014078, NP_054797

UniProt: Q9BYD1