HSPB8 antibody (N-Term)

Catalog No. ABIN2786018

The Rabbit Polyclonal anti-HSPB8 antibody (ABIN2786018) specifically detects HSPB8 in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Guinea Pig and Horse samples.

Quick Overview for HSPB8 antibody (N-Term) (ABIN2786018)

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSPB8 Antibody

Binding Specificity: N-Term

Purpose: HSPB8 Antibody - N-terminal region

Sequence: ADGQMPFSCH YPSRLRRDPF RDSPLSSRLL DDGFGMDPFP DDLTASWPDW

Predicted Reactivity: Cow: 100%, Dog: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HSPB8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSPB8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55035-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HSPB8

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Alternative Name: HSPB8

Background:

Background Information: HSPB8 belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of HSPB8 protein is induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, HSPB8 appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in the encoding HSPB8 gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Heat shock 22 kDa protein 8

Alternative Symbols: H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22

Protein Name: Heat shock protein beta-8

Molecular Weight: 21kDa

Gene ID: 26353

NCBI Accession: NP_055180

UniProt: Q9UJY1