Spartin (SPART) (Middle Region) antibody

Catalog No. ABIN2786078

The Rabbit Polyclonal anti-Spartin antibody (ABIN2786078) specifically detects Spartin in WB. The antibody is reactive with Human, Rat, Mouse, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Dog and Cow samples.

Quick Overview for Spartin (SPART) (Middle Region) antibody (ABIN2786078)

Target: Spartin (SPART)

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: Middle Region

Purpose: SPART Antibody - middle region

Sequence: ASWVSWGLVK GAEITGKAIQ KGASKLRERI QPEEKPVEVS PAVTKGLYIA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against SPG20. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPG20

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55162-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Spartin (SPART)

Alternative Name: SPART

Background:

Background Information: This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome).

Gene Name: spartin

Alternative Symbols: SPG20, TAHCCP1

Protein Name: spartin

Molecular Weight: 73kDa

Gene ID: 23111

NCBI Accession: NP_055902

UniProt: Q8N0X7